Translational_Unit

Part:BBa_K3803011:Design

Designed by: Shiyu Yu   Group: iGEM21_Jiangnan_China   (2021-09-02)


EEVS translational unit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1562
    Illegal BamHI site found at 1081
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The condon of EEVS from Danio rerio had been optimized for better expression in S. cerevisiae.


Source

The promoter and terminator is from S. cerevisiae, and the coding gene is from Danio rerio.

References